DNA Poised for Release in Bacteriophage ø29
نویسندگان
چکیده
Jinghua Tang,1 Norman Olson,1 Paul J. Jardine,2 Shelley Grimes,2 Dwight L. Anderson,2,3,* and Timothy S. Baker1,4,* 1Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology 3Department of Microbiology University of Minnesota, Minneapolis, MN 55455, USA 4Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA *Correspondence: [email protected] (T.S.B.), [email protected] (D.L.A.) DOI 10.1016/j.str.2008.02.024
منابع مشابه
The push-pull mechanism of bacteriophage Ø29 DNA injection.
The mechanism of bacteriophage DNA injection is poorly understood, often considered a simple process, driven merely by the packing pressure inside the capsid. In contrast to the well-established DNA packaging mechanism of Bacillus subtilis phage Ø29, that involves a molecular motor formed by the connector and a viral ATPase, nothing is known about its DNA injection into the cell. We have studie...
متن کاملMolecular Interactions and Protein-Induced DNA Hairpin in the Transcriptional Control of Bacteriophage Ø29 DNA
Studies on the regulation of phage Ø29 gene expression revealed a new mechanism to accomplish simultaneous activation and repression of transcription leading to orderly gene expression. Two phage-encoded early proteins, p4 and p6, bind synergistically to DNA, modifying the topology of the sequences encompassing early promoters A2c and A2b and late promoter A3 in a hairpin that allows the switch...
متن کاملAnalysis of intermolecular base pair formation of prohead RNA of the phage ø29 DNA packaging motor using NMR spectroscopy
The bacteriophage ø29 DNA packaging motor that assembles on the precursor capsid (prohead) contains an essential 174-nt structural RNA (pRNA) that forms multimers. To determine the structural features of the CE- and D-loops believed to be involved in multimerization of pRNA, 35- and 19-nt RNA molecules containing the CE-loop or the D-loop, respectively, were produced and shown to form a heterod...
متن کاملDNA sequence-specific recognition by a transcriptional regulator requires indirect readout of A-tracts
The bacteriophage Ø29 transcriptional regulator p4 binds to promoters of different intrinsic activities. The p4-DNA complex contains two identical protomers that make similar interactions with the target sequence 5'-AACTTTTT-15 bp-AAAATGTT-3'. To define how the various elements in the target sequence contribute to p4's affinity, we studied p4 binding to a series of mutated binding sites. The bi...
متن کاملRamification Amplification: A Novel Isothermal DNA Amplification Method
We have developed a novel isothermal DNA amplification method with an amplification mechanism quite different from conventional PCR. This method uses a specially designed circular probe (C-probe) in which the 3 and 5 ends are brought together in juxtaposition by hybridization to a target. The two ends are then covalently linked by a T4 DNA ligase in a target-dependent manner, producing a closed...
متن کامل